Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint

Abstract

Toxic, mutagenic and carcinogenic heavy metals from tannery industries cause the pollution of agricultural environment and natural water sources. This study aims to isolate, investigate and identify naturally occurring bacteria capable of reducing and detoxifying heavy metals (Chromium, Cadmium and Lead) from tannery effluent. Three isolates were identified up to genus level based on their morphological, cultural, physiological and biochemical characteristics as Gemella sp., Micrococcus sp. and Hafnia sp. Among them Gemella sp. and Micrococcus sp. showed resistance to Lead (Pb), chromium (Cr) and cadmium (Cd), where Hafnia sp. showed sensitivity to cadmium (Cd). All isolates showed different MICs against the above heavy metals at different levels. Degrading potentiality was assessed using Atomic Absorption Spectrophotometer where Gemella sp. and Micrococcus sp. showed 55.16 ± 0.06% and 36.55 ± 0.01% reduction of Pb respectively. On the other hand, moderate degradation of Cd was shown by Gemella sp. (50.99 ± 0.01%) and Micrococcus sp. (38.64 ± 0.06%). Heavy metals degradation capacity of Gemella sp. and Micrococcus sp. might be plasmid mediated, which might be used for plasmid transformation to transfer heavy metal accumulation capability. Therefore, identification of three bacteria for their heavy metal resistance and biodegradation capacity might be a base study to develop the production of potential local bioremediation agents in toxic tannery effluent treatment technology.