SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC DETERMINATION OF 1,4-DIHYDROPYRIDINE DRUGS USING POTASSIUM PERMANGANATE AND CERIUM (IV) AMMONIUM SULPHATE

Abstract

Simple and sensitive spectrophotometric and spectrofluorimetric methods have beendeveloped for determination of 1,4-dihydropyridine (1,4-DHP) drugs based on the oxidation ofthe investigated 1,4-DHP drugs with acidic KMnO4 (method I) or Ce (IV) (method II). The firstmethod is based on the decrease in the colour of the permanganate solution due to the presenceof the studied drug was measured at 525 nm. And the second method is based on monitoring thefluorescence of the produced cerium (III) at emission 355 nm (excitation at 255 nm). Allvariables that affect the performance of the proposed methods were carefully studied andoptimized. The analytical performance of the methods was validated according to InternationalConference of Harmonization guidelines. The proposed methods were applied successfully tothe determination of the drugs in commercial tablets and capsules. The results of the proposedprocedures were statistically and compared with those obtained by the reference methods.