EFFECT OF BIOSYNTHESIZED ZN AND SE NANOPARTICLES ON THE PRODUCTIVITY AND ACTIVE CONSTITUENTS OF GARLIC SUBJECTED TO SALINE STRESS

Abstract

This study is to green synthesize zinc oxide and selenium nanoparticles (ZnO NPs and Se NPs)through aqueous extract of moringa leaves and confirming the synthesis by specialized equipment analysis. In addition, to study the effect of their nanoparticles on the growth, yield, active constituents, and antioxidant activity of garlic plants (Sids 40) under saline stress conditions. The size formed of ZnO NPs was 30-55 nm and Se NPs was 3.5-17 nm. These nanoparticles had a positive effect on all growth and yieldparameters, however; the maximum values were recorded when ZnO NPs applied at 2 ppm. Regarding the active constituents in garlic bulbs, the nanoparticles had an effective role in increasing total phenolic compounds and flavonoids, while Se NPs promoting these compounds, especially at 0.5 ppm. For the GC-MS analysis, it was noted that the major bioactive compounds were about 27. Moreover, nanoparticles had a high effect in increasing the phytoconstituents. As for the antioxidant activity (scavenging DPPH and H2O2), the results showed that the use of nanoparticles led to a high increase in the elimination of these free radicals. Among these, all Se NPs treatments showed the highest potential to scavenge DPPH and H2O2, compared with the control and ZnO NPs. The maximum scavenging of DPPH and H2O2were recorded by Se NPs at 2 and 1 ppm, respectively.